FIGURE 3.

Investigation of factors affecting the n‐propanol/NaOH cleaning in place strategy using RoboColumns by purifying clarified cell culture harvest (CCCH) and analyzing the generated resin extracts: (A) Relative host cell protein (HCP) content in Capto Core 700 resin extracts compared to HCP content in CCCH as a function of n‐propanol and NaOH concentrations in cleaning in place solution; (B) SDS‐PAGE analysis of the resin extracts analyzed in (A). In (B), the top box denotes the samples analyzed and the bottom box denotes the conditions leading to the corresponding analyzed resin extracts