Table 3. Primers.
| Gene | Primer sequence (5___>3) |
|---|---|
| βactin_F | CAACACAGTGCTGTCTGGTGGTA |
| βactin_R | ATCGTACTCCTGCTTGCTGATCC |
| IL4_F | TGTGCCCACGCTGTGCTTACA |
| IL4_R | CTTGTGGCAGTGCTGGCTCTCC |
| IL6_F | AGAAATCCCTCCTCGCCAAT |
| IL6_R | AAATAGCGAACGGCCCTCA |
| IFNγ_F | CTGAAGAACTGGACAGAGAG |
| IFNγ_R | CACCAGCTTCTGTAAGATGC |
| HSP27_F | GGAGATCACCGGCAAACACG) |
| HSP27_R | CCTCCACTGTCAGCATCCCA |
| TLR4_F | GTCTCTCCTTCCTTACCTGCTGTTC |
| TLR4_R | AGGAGGAGAAAGACAGGGTAGGTG |
| TNF-α | CCCCCAGAAGGAAGAGTTTC |
IL, interleukin; IFN, interferon; HSP, heat shock protein; TLR, toll-like receptor; TNF, tumor necrosis factor.