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. 2022 Jul 27;14(9):e15904. doi: 10.15252/emmm.202215904

Figure EV1. ACE2 proteins are detected in the serum exosomes of all six COVID‐19 patients.

Figure EV1

The identified peptide sequences (red color) of endogenous ACE2 by mass spectrometry analyses using exosomes isolated from the sera of six COVID‐19 patients and six healthy controls (Cohort #3). All six COVID‐19 patients but only two of six healthy controls showed exosomal ACE2 proteins in the serum exosomes. Exosomes were sequentially isolated using ExoQuick kits and then ExoQuick ULTRA columns. Exosomes were further purified by immunoprecipitation using a combination of anti‐CD9, anti‐CD63, and anti‐CD81 magnetic beads. The protein score is the sum of the highest ions score of MS/MS search for each distinct peptide.