Figure 2. The upregulated genes in GLK‐positive epithelial cells belong to the pathways of intracellular/extracellular vesicle and viral process.

• A–D
  Endogenous GLK levels were increased by the SARS‐CoV‐2 spike protein. Immunoblotting analysis of the endogenous GLK and vinculin proteins from the lysates of HCC827 lung epithelial cells either infected with SARS‐CoV‐2 pseudovirus (A) or treated with the SARS‐CoV‐2 spike protein (C). Real‐time PCR analysis of mouse GLK mRNA levels in HCC827 lung epithelial cells either infected with SARS‐CoV‐2 pseudovirus (B) or treated with the SARS‐CoV‐2 spike protein (D). The mRNA levels of GLK were normalized to GAPDH mRNA levels. n = 2 (technical replicates) per group.
• E–H
  KEGG (Kyoto Encyclopedia of Genes and Genomes)‐enriched pathways of the upregulated genes in GLK‐positive KRT18⁺ epithelial cells or KRT18⁺ TPPP3⁺‐ciliated epithelial cells from Cohort #1 (E and F) and Cohort #2 (G and H). Pathways belonging to different classifications are listed on the left side of the plot. Varied numbers of genes enriched in individual pathways are presented by different diameter sizes and numbers for individual dots. Adjusted P‐value is ranging from 0 ~ 1; less P‐value means greater intensiveness.

Data information: In (B, D), data are presented as mean values. Data shown are representative results of three (A–D) independent experiments.

Source data are available online for this figure.