TABLE 1.
GAT and DHAPAT activities in subcellular fractions of wild-type strain and the gat1 mutant strain TTA1
| Fraction | DBY746 (wild type)
|
TTA1 (gat1)
|
||
|---|---|---|---|---|
| Relative specific activity ofa:
|
Relative specific activity ofa:
|
|||
| GAT | DHAPAT | GAT | DHAPAT | |
| Homogenate | 1 (0.68 ± 0.20) | 1 (0.15 ± 0.04) | 1 (0.05 ± 0.01) | 1 (0.03 ± 0.01) |
| Lipid particles | 31.0 | 9.3 | Trace | NDb |
| Microsomes (30,000 × g) | 5.8 | 2.4 | 4.0 | 1.3 |
| Microsomes (40,000 × g) | 5.0 | 0.9 | 1.2 | 0.4 |
| Mitochondria | 1.5 | 2.6 | 0.9 | 1.0 |
a
Mean values from three independent experiments are shown; the mean deviation was ±15%. Specific activities (nanomoles per milligram per min) are shown in parentheses.
b
ND, not detectable.