TABLE 2.
3H/14C ratios in different glycerolipids after labeling of wild-type and acyltransferase mutant strains with [2-3H, U-14C]glycerol
| Glycerolipid |
3H/14C ratio after labeling ofa:
|
||||
|---|---|---|---|---|---|
| DBY746 (wild type) | TTA1 (gat1) | SJ21R (wild type) | YMN5 (slc1) | gat1slc1 | |
| Phosphatidic acid | 3.82 | 2.99 | 2.47 | 2.22 | 2.96 |
| Phosphatidylcholine | 3.47 | 3.02 | 2.80 | 2.09 | 2.79 |
| Phosphatidylethanolamine | 3.67 | 3.02 | 2.77 | 1.99 | 2.66 |
| Phosphatidylinositol | 3.42 | 3.02 | 2.64 | 2.12 | 2.86 |
| Phosphatidylserine | 3.72 | 3.35 | 2.60 | 2.10 | 3.10 |
| Cardiolipin | 3.16 | 2.50 | 2.13 | 1.73 | 2.45 |
| Diacylglycerols | 3.57 | 2.96 | 3.24 | 2.28 | 2.48 |
| Triacylglycerols | 3.58 | 3.10 | 3.07 | 2.24 | 3.55 |
a
Double labeling experiments with [2-3H, U-14C]glycerol were carried out as described in the Materials and Methods section. Decrease of the 3H/14C ratio is indicative of increased contribution of the DHAP pathway to glycerolipid formation. Experiments were carried out in triplicate; the mean deviation was ±5%.