Figure 6.

Summary of enriched genes and acquisition of drug resistance of cancer cells during bTME+ co‐culture. a) Cytoscape network map. Each node or circle represents a specific pathway that is enriched in either BM‐NSCLC, Astrocytes, or bECs. Red color indicates enrichment of the corresponding pathway and the node is divided into 3 separate segments, each segment reflecting activation of the pathway in BM‐NSCLCs, Astrocytes, or bECs. The size of the node reflects the number of genes that constitute each pathway, meaning that bigger nodes are comprised more genes compared to the smaller nodes. Last, two or more nodes are connected by an edge or a line if they share at least one gene in common. b) cellular responses to drug treatment and bTME. Black‐dashed lines indicate cellular pathways related to targeted drugs; red‐dashed lines indicate up‐regulated pathways induced by cytokines from bTME. The tables summarize drug screening results representing gene‐based prediction and cell‐based testing results in the microfluidic chip (G: gene‐based prediction, C: drug screening result without bTME, bTME: drug screening results with bTME. S: sensitive response, U: unknown, R: resistive response, red box: BM‐ADC, blue box: BM‐SqCC). Green arrows and characters mean increased cytokines and chemokines in bTME+. Red arrows and characters indicate increased genesets in bTME+.