Figure 2.

2D PDCCs‐derived 3D ALI organotypic cultures and xenografts retain histologic features of their parental tumors. A) Representative H&E staining images of 3D ALI organotypic cultures, xenografts, and matched primary tumors. Scale bar: 50 µm. B) Brightfield images of NSCs derived from noncancer tissues in three patients. Scale bar: 50 µm. H&E staining of corresponding ALI organotypic cultures. Scale bar: 50 µm. C) Concordant expression of MUC2 and KI67 in primary tumors and their derived ALI organotypic cultures and xenografts. Scale bar: 50 µm. D) Brightfield, histological and immunohistochemically images of spheroids derived from PDCCs. Scale bars: 50 µm.