FIG. 3.

Overexpression of the Msu proteins. The Msu proteins were overexpressed in E. coli BL21(DE3) containing various pET24b-derived constructs, as described in Materials and Methods. Total cell extracts were separated by SDS-polyacrylamide gel electrophoresis (12% gel), and the proteins were visualized with Coomassie blue. The positions of the MsuE, MsuD, and MsuC proteins are indicated. Lanes: M, molecular weight marker; 1, BL21(pET24b); 2, BL21(pME4503); 3, BL21(pME4529). Approximately 10 μg of protein was loaded per lane.