TABLE 3.
Substrate spectrum of NADH-dependent flavin reductase
| Nucleotide | Flavin reductase activity (μmol/min/mg of protein)a with:
|
|||
|---|---|---|---|---|
| FMN | FAD | Riboflavin | Lumiflavin | |
| NADH | 1.85 | 0.83 | 0 | 0 |
| NADPH | 0.07 | 0.03 | 0 | 0 |
a
Flavin reductase activity in extracts of overexpressed MsuE (plasmid pME4531) was measured as described in Materials and Methods. No activity was seen with a similar extract prepared with cells containing pET24b. The values given were obtained after correction for the reduction rates of NADH and NADPH in the absence of flavin, which were 0.20 and 0.013 μmol/min/mg, respectively.