TABLE 4.

Substrate spectrum of desulfonation by the MsuED enzymes

Substrate	Desulfonation rate (%)a
Methanesulfonate	100
PIPESb	31 ± 2
Ethanesulfonate	27 ± 3
Isethionate	19 ± 2
MOPSc	17 ± 2
Pentanesulfonate	13 ± 2
HEPES	9 ± 0.7
Sulfoacetate	1.8 ± 0.7
Taurine	1.5 ± 0.7
Allylsulfonate	1.3 ± 0.9
Cysteate	0
Sulfate	0
Benzenesulfonate	0
Dimethyl sulfoxide	0
Methionine	0

^aDesulfonation was measured with cell extracts of E. coli BL21(pME4503) as described in Materials and Methods. The results are the averages and standard deviations of three determinations and are normalized to the values obtained with methanesulfonate as the substrate (100% = 35 ± 2.5 nmol/min/mg of protein). 

^bPIPES, piperazine-N,N′-bis(2-ethanesulfonic acid). 

^cMOPS, morpholinepropanesulfonic acid.