TABLE 5.
Expression of msuD::xylE and atsA in strain SLF3a
| Sulfur source for growth (250 μM) | Catechol-2,3-dioxygenase activity (μmol/min/mg of protein) | Arylsulfatase activity (nmol/min/mg of protein) |
|---|---|---|
| Sulfate | 0.6 ± 0.2 | 0.29 ± 0.04 |
| Cysteine | 47 ± 4 | 1.72 ± 0.03 |
| Methionine | 473 ± 5 | 17.7 ± 1.0 |
| Pentanesulfonate | 118 ± 12 | 14.6 ± 0.9 |
| Pentanesulfonate + sulfate | 2.0 ± 1.4 | 0.27 ± 0.04 |
a
Cells were harvested in mid-exponential phase, and catechol oxygenase and arylsulfatase activities in cell extracts were measured as described in Materials and Methods. The results represent means and standard deviations from four measurements.