Skip to main content
. 2022 Jul 28;24(3):603. doi: 10.3892/etm.2022.11540

Figure 4.

Figure 4

Effect of ROCK2 silencing on the NF-κB/NLRP3 pathway in LPS-induced HK-2 cells. (A) The protein expression of p-NF-κB p65/NF-κB p65, NLRP3, ASC, caspase-1 p20/pro-caspase-1 in HK-2 cells in different groups was measured using western blotting. (B) PMA was used to treat LPS-induced, shRNA-ROCK2-transfected HK-2 cells or untransfected cells, then the protein expression levels of p-NF-κB p65/NF-κB p65, NLRP3, ASC, caspase-1 p20/pro-caspase-1 in HK-2 cells in different groups were measured using western blotting. ***P<0.001 vs. control; $$$P<0.001 vs. LPS; ###P<0.001 vs. LPS + shRNA-ROCK2 or LPS + shRNA-NC; ++P<0.01 and +++P<0.001 vs. DMSO + LPS + shRNA-ROCK2. ROCK2, Rho-associated protein kinase 2; LPS, lipopolysaccharide; sh, short hairpin; NC, negative control; NLRP3, NLR family pyrin domain containing 3; ASC, apoptosis-associated speck-like protein containing a CARD; PMA, phorbol 12-myristate 13-acetate; p, phosphorylated; t, total.