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. 2022 Jul 22;10:933220. doi: 10.3389/fcell.2022.933220

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Copyright © 2022 Akiyama-Oda, Akaiwa and Oda.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of the cumulus on the UMAP plot. (A) Expression of LOC107444265 (Pt-noggin-D) in a germ disc at late stage 5. The signal is detected at the cumulus. The right panel shows DAPI staining of the same embryo. (B) Expression of LOC107444265 visualized on the UMAP plot. The region boxed in yellow was used as a template in the Open CV similarity search after the background color was changed to the color of cells without expression as in (B′). (C) The list of genes with highest similarity scores in the similarity search. (D) Expression of the top 6 genes except LOC107444265 visualized on the UMAP plot and detected using WISH. In situ hybridization of LOC107455782 was not performed. Scale Bars = 200 μm.