FIGURE 4.

Lipid droplet (LD) accumulate in NPC cells. NF-κB is associated with LDs in NPC cells. (A) Immunofluorescent analysis of lipid content in NPE cell lines (NP69 and NP460) and NPC cell lines (HONE1 and HK1) USING a lipid-specific fluorescent dye BODIPY 493/503. (B) Cells were treated with 10µg/mL of PGN for 2 hours, the media was changed, and the cells were harvested at 0 and 4 h time points. The HK1 versus NP69 cells were fractionated on a discontinuous density gradient to separate lipid-containing fractions. Fractions were analyzed using Western blot for the expression of NF-κB (p65).