FIG. 6.

Influence of rifampin on induction of nitrite reductase activity after a shift from aerobiosis to anaerobiosis. After aerobic precultivation without (circles) or with (triangles) nitrite, no antibiotic or 200 μM rifampin was added to equal parts of each culture and the incubation was continued for 15 min. Subsequently, the remaining nitrite was removed by centrifugation and two washing steps. Cells were resuspended in modified BM with or without rifampin and incubated anaerobically at 37°C. After addition of nitrite, samples were taken at different time points and the amount of nitrite was monitored.