Figure 1.

Preferential penetration of p28 into human glioblastoma cells. (A), human GBM cell lines (U-118 and LN-229) and astrocyte and fibroblast were cultured with Alexa Fluor 568–labeled p28 at 37°C for 2 hr, and images were recorded by confocal microscopy. Red, Alexa Fluor 568–labelled p28; blue, DAPI (nucleus). (B), Human GBM (LN-229 and U-118) and fibroblast were treated with ICG-p28 for 2 hr. Lysates (20 µg/lane) were separated by 10% Native PAGE and ICG-p28 was imaged by the Odyssey imaging system with an 800nm channel. Positive control: 10, 20, and 50 ng/lane of pure ICG-p28. +: ICG-p28 treated cell lysate. -: untreated cell lysate.