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. 2022 Jul 22;12:927706. doi: 10.3389/fonc.2022.927706

Figure 11.

Figure 11

TNFRSF21 was a downstream target of miR-193a-3 and lncRNA MIR31HG sponged miR-193a-3p to upregulate TNFRSF21. (A) TNFRSF21 expression in lung adenocarcinoma (LUAD) specimens compared with normal tissues as detected by qRT-PCR. *** p < 0.001 vs. normal tissue. (B) TNFRSF21 expression in 6 LUAD patients relative to normal tissues as detected by western blot. (C, D) TNFRSF21 expression in different LUAD cell lines (A549, NCI-H2009, and PC9) relative to bronchial epithelioid cell (HBE) was estimated by qRT-PCR or western blot. *** p < 0.001 vs. HBE. (E, F) TNFRSF21 expression following miR-193a-3p overexpression was assessed by qRT-PCR or western blot. ** p < 0.01; *** p < 0.001 vs. ctrl mimics. (G) Schematic diagram of the predicted interacting sites. (H, I) The relationship between TNFRSF21 and miR-193a-3p in NCI-H2009 and A549 cells was performed by dual-luciferase reporter assay. ** p < 0.01 vs. ctrl mimics. (J) Relationship between TNFRSF21 and miR-193a-3p investigated by Pearson’s analysis. (K, L) TNFRSF21 expression following si-MIR31HG or si-MIR31HG + miR-193a-3p inhibitor was assessed by qRT-PCR or western blot. *** p < 0.001 vs. ctrl inhibitor; ## p < 0.01 vs. si-MIR31HG + miR-193a-3p inhibitor. (M) Schematic diagram of the mechanism of lncRNA MIR31HG/miR-193a-3/TNFRSF21 regulatory axis.