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. 2022 Jul 22;12:927706. doi: 10.3389/fonc.2022.927706

Figure 9.

Figure 9

lncRNA MIR31HG acted as ceRNA for miR-193a-3p. (A) miR-193a-3p expression in lung adenocarcinoma (LUAD) specimens compared with normal tissues as detected by qRT-PCR. *** p < 0.001 vs. normal tissue. (B) miR-193a-3p expression in different LUAD cell lines (A549, NCI-H2009, and PC9) relative to bronchial epithelioid cell (HBE) was estimated by qRT-PCR. (C) miR-193a-3p expression following si-MIR31HG transfection was assessed by qRT-PCR. (D) MIR31HG expression following miR-193a-3p overexpression was measured by qRT-PCR. (E) Schematic diagram of the predicted interacting sites. (F, G) The relationship between MIR31HG and miR-193a-3p in NCI-H2009 and A549 cells was performed by dual-luciferase reporter assay. (H, I) The immunoprecipitation of MIR31HG and miR-193a-3p in NCI-H2009 and A549 cells was determined by RNA immunoprecipitation experiment. (J) The relationship between MIR31HG and miR-193a-3p was investigated by Pearson’s analysis. ** p < 0.01; *** p < 0.001.