TABLE 1.
Primers used for construction of probes by PCR
| Genea | Sequence
|
|
|---|---|---|
| Upstream primer | Downstream primer | |
| acsA | 5′-TTCCGCGATCTCTCCCATAA-3′ | 5′-GAAACGGCAGCAAATACCTT-3′ |
| ascB | 5′-CCAGAAGGTAAAGAGCCGGT-3′ | 5′-AACAGCGAATAACCGGCAGG-3′ |
| acsC | 5′-CCTTTGACGGGACTGGAGAT-3′ | 5′-GGCGCCCAGGACTACCTTGG-3′ |
| ascD | 5′-GCCGTCCAGATTTTACGTGA-3′ | 5′-GGGCCAGTCGGGTACGATAT-3′ |
| acsE | 5′-CTCATTATCGGTGAACGGAT-3′ | 5′-GCTGACCTCCTGGGTGACTC-3′ |
a
acsA through acsE encode the β and α subunits of CODH, the α and β subunits of CFeSP, and MeTr, respectively.