TABLE 2.
Induction of polysaccharide and Pck activity in mutant M. xanthus strainsa
| Strainb | Specific carbohydrate content (μg of carbohydrate/mg of protein)
|
Pck sp act (nmol/min/mg of protein)
|
||
|---|---|---|---|---|
| Log | Stationary | Log | Stationary | |
| DK1622 (WT) | 72 (5) | 127 (9) | 8.1 (1.8) | 18.1 (2.5) |
| LS1102 (stk) | 153 (11) | 169 (13) | 18.6 (3.0) | 17.6 (2.7) |
| DZF1 (sglA1) | 64 (2) | 60 (2) | 6.3 (2.0) | 13.6 (2.8) |
| JD300 (esg) | 61 (3) | 56 (2) | 6.7 (1.6) | 15.2 (3.2) |
| JD509 (esg stk) | 122 (9) | 119 (11) | 12.3 (2.7) | 12.1 (2.3) |
| DK3468 (dsp) | 66 (3) | 67 (2) | 7.6 (2.0) | 22.3 (4.6) |
| LS1111 (dsp stk) | 81 (4) | 74 (3) | 7.6 (1.6) | 25.2 (5.2) |
| DK3481 (sgl) | 73 (3) | 70 (2) | 8.3 (2.2) | 16.8 (3.7) |
| LS1118 (sgl stk) | 130 (8) | 134 (6) | 13.9 (3.0) | 13.8 (3.2) |
| DK3482 (tgl) | 57 (2) | 57 (3) | 5.0 (1.5) | 21.0 (3.8) |
| SR53 (Cds) | 62 (3) | 60 (2) | 5.3 (1.7) | 16.1 (3.3) |
| JD510 (Cds stk) | 134 (10) | 136 (12) | 14.3 (2.6) | 14.1 (2.9) |
| SR171 (Cds) | 67 (2) | 69 (3) | 7.6 (2.1) | 18.4 (3.2) |
| JD511 (Cds stk) | 91 (5) | 94 (8) | 11.6 (3.1) | 12.1 (2.9) |
| SR200 (Cds) | 60 (2) | 57 (2) | 5.9 (1.7) | 12.7 (2.5) |
| JD512 (Cds stk) | 70 (3) | 68 (3) | 6.5 (1.8) | 14.2 (2.0) |
a
All measurements were made by using concentrated cell suspensions obtained from log-phase CYE cultures of the various strains. The determinations of specific carbohydrate content and Pck activity were from cell suspensions either immediately after concentration (Log) or after 6 h of incubation in CYE shaker culture (Stationary). The values reported for the specific carbohydrate content and Pck activity are the averages of measurements from three independent experiments. The standard errors are shown in parentheses.
b
Strains are indicated by strain numbers (Table 1) with genotypic and/or phenotypic designations in parentheses.