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. 2022 Jul 22;13:948457. doi: 10.3389/fimmu.2022.948457

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Copyright © 2022 Zhu, Xing, Zhang, Zhang, Shen, Chen, Shen, Jiang, Yuan, Zhang, Wang, Wu, Zhou, Wu, Deng, Liu, Zhang and Yang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Generation of AAVs that specifically deplete IL-10 in microglia. (A) Generation of an AAV PHP.B serotype carrying siRNA for IL-10 (si-IL-10) or control scrambled sequence (Scr) under a microglia-specific TMEM119 promoter, which allows specific depletion of IL-10 in microglia. T2A allowed co-expression of transgenes with a GFP reporter under the same promoter. (B) Scr virus was used to infect four different cell lines, which were microglia cell line HMC3, macrophage cell line KG-1, neuronal cell line HCN-1, and fibroblast cell line 3T3. Bright fields and GFP channel were shown. (C) Si-IL-10 and control Scr viruses were used to infect HMC3 cells. Bright fields and immunocytochemistry for IL-10 were shown. (D) ELISA for IL-10. *p < 0.05. N = 5. Scale bars are 100 µm.