Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jul 22;13:939090. doi: 10.3389/fphar.2022.939090

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Chen, Guo, Zhao, Xu, Song, Yu, Pilarsky, Nainu, Li, Zhou and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Methods of CRISPR/Cas9 technology to construct animal models. (A) Editing gene in embryos. Collecting the zygote that was injected with Cas9 mRNA, sgRNA, and DNA. After the zygote develops into an embryo, it would be transferred into the animals to produce generation. (B) Editing gene in haploid ESCs and SSCs: haploid embryonic stem cells (ESCs) are pluripotent cells generated from oocytes. CRISPR/Cas9-mediated gene editing in ESCs and autologous transplant to pseudo-pregnant animals to produce generation. Spermatogonia stem cells (SSCs) would be transfected by RNP and autologous transplant to seminiferous tubule to produce the generation.