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. 2022 Jul 22;13:919940. doi: 10.3389/fphar.2022.919940

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Copyright © 2022 Lin, Ge, Tang, He, Moqbel, Xu, Ma, Zhou, Ran and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NitC reduced ROS generation and ameliorated cellular senescence in RACs. (A) The potential targets of NitC were obtained by PharmMapper. The protein-protein association network of the targets was established by STRING. (B) The top 10 hub genes were calculated by Cytoscape subsequently. Genes marked with “ROS” are involved with the modulation of ROS generation according to the existing research. (C) Immunofluorescent microscopy was utilized to detect the ROS in RACs. The intensity of fluorescence represented the relative level of ROS. (D) The ROS levels of different groups were also assessed through flow cytometry. (E) β-Galactosidase activity assay was performed in RACs. Typical normal RAC and senescent RAC were shown. Red arrows mark the senescent RAC. (F) The WB analysis of cellular senescence-related genes: p16 and p53 were conducted. Meanwhile, the quantified results of relative protein expression were present. #p < 0.05 versus the control group. ∗p < 0.05 versus the model group. Scale bars = 50 μM.