FIG. 1.
ToxT-dependent expression of CT and TCP is modulated by temperature and bile in a V. cholerae wild-type strain. V. cholerae O395 carrying plasmid pKEK162, which expresses ToxT from the Plac promoter, was grown as described in Materials and Methods in LB containing 0.3 mM IPTG in the absence (−) or presence (+) of 0.4% bile at 30 or 37°C. Arrows indicate agglutinated cells resulting from TCP expression. CT levels in culture supernatants were determined as described in Materials and Methods.