. 2022 Jul 22;10:907589. doi: 10.3389/fbioe.2022.907589

TABLE 2.

Characteristics of in vivo studies.

In vivo studies	Aplicability	Cost	Difficulty of coating preparation	Substrate	Coating and method	Outcomes
Zhang et al. (Zhang et al., 2020)	+ +	+ + +	+ + +	Porous Ti	Cht/HA composite coating	Osteoblast-like cell proliferation and differentiation
Zhang et al. (Zhang et al., 2020)	+ +	+ + +	+ + +	Porous Ti	Electrochemical deposition	Osteoblast-like cell proliferation and differentiation
Takanche et al. (Takanche et al., 2018)	+	+ + +	+ + +	Ti	Chitosan-gold nanoparticles	Increased osteogenesis and inhibition of osteoclastogenesis
Takanche et al. (Takanche et al., 2018)	+	+ + +	+ + +	Ti	Graft-on technique	Increased osteogenesis and inhibition of osteoclastogenesis
Bumgardner et al. (Bumgardner et al., 2007)	+ +	+ +	+ +	Ti	1 wt% of 92.3% deacetylated chitosan in 1% acetic acid	Minimal inflammatory response and development of lamellar bone
Bumgardner et al. (Bumgardner et al., 2007)	+ +	+ +	+ +	Ti	Solution casting
Kung et al. (Kung et al., 2011)	+	+ +	+ +	Ti	Two types of chitosan with molecular weights of 450 and 750 kDa and with deacetylation degree	New bone formed ectopically
					> 90%
					Enveloped membranes
López-Valverde et al. (López-Valverde et al., (2021a); López-Valverde et al., (2022))	+ + +	+	+	Porous	Pure chitosan acid solution	Higher bone density compared to the conventional etched surface group
	+ + +	+	+	Ti6Al4V	Immersion

+ low; + + moderate; + + + high; HA, hydroxyapatite.