Table 1.

Antigen-binding domains tested as CAR elements in this study.

Antibody Name	Type	Target	Affinity (K D)	Cross-Reactivity	Competition	Epitope Location	Ref
sdAb021	sdAb	EGFR (Erbb1)	38.5 nM	Human, Cyno	Competitive with sdAb027, partial with sdAb028	Unknown, likely domain IV	(29)
sdAb027	sdAb	EGFR (Erbb1)	1.6 nM	Human, Cyno	Competitive with sdAb021, partial with sdAb028	Unknown, likely domain IV	(29)
sdAb028	sdAb	EGFR (Erbb1)	9.0 nM	Human, Mouse	Partial competition with sdAb021 and sdAb027	Unknown, likely domain IV	(29)
Trastuzumab	scFv	HER2 (Erbb2)	n.d. (5 nM) a	Human, Cyno	Juxtamembrane conformational epitope in domain IV	Very membrane proximal	(30, 31)
F265	scFv	EGFRvIII	n.d. (27.5 nM) a	Unknown	Domain I/III neoepitope, non-competitive with F269	Membrane distal	(32) and unpublished
F269	scFv	EGFRvIII	n.d. (31.5 nM) a	Unknown	Domain I/III neoepitope of EGFR, non-competitive with F265	Membrane distal	(32) and unpublished

^aBinding of scFvs was not evaluated. Monovalent affinity of full-length IgG is shown in parentheses.

Cyno, cynomolgus monkey.