Table 2. Dissociation Constants of LmrR Variants with Dau and RBF.
Binding
affinity, Kd (nM) (Mean ± SD)a |
|||
---|---|---|---|
LmrR protein | Dau at 10 °C | Dau at 25 °C | RBF at 25 °C |
5MeW | 104 ± 5 | 370 ± 30 | 380 ± 8 |
W | 315 ± 40 | 450 ± 50 | 460 ± 50 |
5FW | 280 ± 40 | 430 ± 80 | 970 ± 50 |
5,6diFW | 560 ± 50 | 1050 ± 60 | 4990 ± 60 |
5,6,7triFW | 590 ± 60 | 1440 ± 100 | 8370 ± 200 |
4,5,6,7tetraFW | 1680 ± 70 | 2880 ± 500 | 33400 ± 16000 |
“SD”, standard deviation (n = 3).