Table 3. Experimental Free Energy ΔG of Ligand Binding to LmrR Variants and Free Energy Difference ΔΔG of Dau Binding to LmrR Variants between at 10 and 25 °C.

	Dau (kJ/mol)		ΔΔG Dau (kJ/mol)	RBF (kJ/mol)
LmrR protein	ΔG10 °C	ΔG25 °C	ΔG10 °C – ΔG25 °C	ΔG25 °C
5MeW	–37.8	–36.7	–1.1	–36.6
W	–35.2	–36.2	0.9	–36.2
5FW	–35.5	–36.4	0.9	–34.3
5,6diFW	–33.9	–34.1	0.2	–30.2
5,6,7triFW	–33.7	–33.3	–0.4	–29.0
4,5,6,7tetraFW	–31.3	–31.6	0.3	–25.7