TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype | Reference or source |
|---|---|---|
| Strains | ||
| CY15076 | W3110 tnaA2 ΔlacU169 | This study |
| PDG1158 | SVS1100 (λtnap tnaC-UAA-tnaA′-′lacZ) | 17 |
| PDG1184 | SVS1100 (λtnap tnaC-UUA-tnaA′-′lacZ) | 17 |
| SVS1100 | W3110 bglR55Δ (lac-argF)U169 | 46 |
| SVS1144 | SVS1100 (λtnap tnaA′-′lacZ) | 46 |
| VK100 | SVS1100 (λtnap tnaC-UGA-′lacZ) | 28 |
| VK100/SerUUGA | VK100 serU [Ser UG(A/G)] suppressor | 28 |
| VK700 | CY15076 (λtnap tnaC-UAG-′lacZ) | This study |
| VK700/SerUUAG | VK700 serU (Ser UAG suppressor) | This study |
| VK701 | VK700 prfA1(Ts) | This study |
| VK800 | CY15076 (λtnap tnaC-UAG-tnaA′-′lacZ) | This study |
| VK801 | VK800 prfA1(Ts) | This study |
| VK805 | VK801 trpA9761 | This study |
| VK900 | CY15076 (λtnap tnaC-UAA-′lacZ) | This study |
| VK1100 | CY15076 (λtnap tnaC-UAG UUUGAC-tnaA′-′lacZ) | This study |
| VK1101 | VK1100 prfA1(Ts) | This study |
| VK1145 | SVS1144 prfA1(Ts) | This study |
| VK1159 | PDG1158 prfA1(Ts) | This study |
| VK1185 | PDG1184 prfA1(Ts) | This study |
| VK1200 | CY15076 (λtnap tnaC-UAG UUGACC-tnaA′-′lacZ) | This study |
| VK1201 | VK1200 prfA1(Ts) | This study |
| Plasmids | ||
| pGFIBI | hisT su (UAG suppressor) | 35 |
| pMY228 | trpT su7 (UAG suppressor) | 36 |
| pRS552 | pBR322 derivative, lac-based vector | 44 |
| pSWC115 | trpT su7 (UAA suppressor) | 36 |