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. 2022 Aug 5;13:92. doi: 10.1186/s40104-022-00739-7

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — RS diet suppresses osteoclastic bone resorption in meat ducks. a Tartrate resistant acid phosphatase (TRAP) staining of tibial sections. Bar = 100 μm. b The number of osteoclast (N.Oc/BS) in proximal tibias was determined by histomorphometry. Circulating (c) TRAP activity and (d) C-terminal cross-linked telopeptide of type I collagen (CTx) concentrations. (e) Real-time RT-PCR analysis for mRNA expression of receptor activator for nuclear factor-κB ligand (RANKL) and osteoprogerin (OPG) in the proximal end, and the ratio of RANKL/OPG was calculated. Serum bone formation including (f) procollagen type I N-terminal propeptide (P1NP) and (g) alkaline phosphatase (ALP), as well as (h) serum calcium (Ca) and phosphorus (P) were evaluated. Data are expressed as mean and standard deviation. ^a,bMean values with different letters are significantly different by one-way analysis of variance followed by Tukey’s post hoc test (P < 0.05)