TABLE 2.
Expression of plasmid-borne nolA-lacZ fusions in B. japonicum wild-type (USDA 110) and nolA mutant strains
| Strain | Phenotype (chromosomal) | β-Galactosidase activity (U)a
|
|
|---|---|---|---|
| Uninduced | + SSG | ||
| nolA1-lacZ fusion on pNMAlac23 | |||
| USDA 110 | Wild type | 15 ± 3 | 292 ± 15 |
| BjB3 | NolA− | 18 ± 4 | 250 ± 18 |
| BJL823 | NolA1 | 16 ± 7 | 180 ± 15 |
| BJL813 | NolA2 | 17 ± 6 | 238 ± 16 |
| BJL812 | NolA3 | 26 ± 4 | 71 ± 10 |
| BJL83 | NolA1, NolA2 | 19 ± 5 | 255 ± 12 |
| BJL82 | NolA1, NolA3 | 19 ± 4 | 140 ± 12 |
| BJL81 | NolA2, NolA3 | 20 ± 4 | 94 ± 3 |
| BJL123 | Null (Δ123) | 19 ± 10 | 220 ± 20 |
| nolA2-lacZ fusion on pNMAlac13 | |||
| USDA 110 | Wild type | 20 ± 4 | 150 ± 18 |
| BjB3 | NolA− | 25 ± 6 | 52 ± 4 |
| BJL823 | NolA1 | 18 ± 2 | 149 ± 3 |
| BJL813 | NolA2 | 23 ± 6 | 62 ± 6 |
| BJL812 | NolA3 | 17 ± 5 | 45 ± 5 |
| BJL83 | NolA1, NolA2 | 16 ± 5 | 80 ± 8 |
| BJL82 | NolA1, NolA3 | 16 ± 5 | 88 ± 11 |
| BJL81 | NolA2, NolA3 | 18 ± 2 | 56 ± 13 |
| BJL123 | Null (Δ123) | 19 ± 4 | 42 ± 5 |
| nolA3-lacZ fusion on pNMAlac12 | |||
| USDA 110 | Wild type | 25 ± 6 | 250 ± 19 |
| BjB3 | NolA− | 32 ± 7 | 56 ± 8 |
| BJL823 | NolA1 | 16 ± 6 | 228 ± 3 |
| BJL813 | NolA2 | 17 ± 6 | 73 ± 10 |
| BJL812 | NolA3 | 26 ± 4 | 53 ± 8 |
| BJL83 | NolA1, NolA2 | 16 ± 6 | 270 ± 20 |
| BJL82 | NolA1, NolA3 | 19 ± 4 | 87 ± 8 |
| BJL81 | NolA2, NolA3 | 20 ± 5 | 71 ± 5 |
| BJL123 | Null (Δ123) | 11 ± 3 | 57 ± 20 |
a
Units with CPRG as a substrate. Values are the means of two independent determinations. The standard deviation is indicated.