TABLE 3.
Nodulation phenotype of B. japonicum USDA 438 and its transconjugants on G. max PI 377578 and G. max cv. Kasota
| Strain | NolA expressed | No. of nodules/planta
|
|
|---|---|---|---|
| PI 377578 | cv. Kasota | ||
| nolA from wild-type promoter | |||
| USDA 438 | |||
| + pRK290 | None | 2 ± 1 | 50 ± 11 |
| + pJLDA | NolA1, NolA2, NolA3 | 46 ± 6 | 53 ± 17 |
| + pJLDA23 | NolA1 | 27 ± 7 | 48 ± 9 |
| + pJLDA13 | NolA2 | 1 ± 1 | 52 ± 5 |
| + pJLDA12 | NolA3 | 2 ± 1 | 51 ± 5 |
| + pJLDA123 | Null (Δ123) | 2 ± 0 | 39 ± 6 |
| nolA from trp promoter | |||
| USDA 438 | |||
| + pTE3 | None | 1 ± 1 | 54 ± 7 |
| + pTE3A | NolA1, NolA2, NolA3 | 23 ± 5 | 37 ± 11 |
| + pTE3A23 | NolA1 | 0 | 32 ± 4 |
| + pTE3A13 | NolA2 | 0 | 45 ± 4 |
| + pTE3A12 | NolA3 | 27 ± 1 | 52 ± 13 |
| + pTE3A123 | Null (Δ123) | 0 | 42 ± 1 |
a
Nodule numbers per plant were compared 30 days after inoculation. Values are means for three plants ± standard error of means.