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SDS-PAGE (A) and zymogram (B) analyses of the FlgJ proteins with amino acid substitutions in the putative active center of muramidase. The procedures used were the same as those described in the legend to Fig. 3. The arrows indicate the positions of the His-tagged FlgJ proteins. The proteins used were His-FlgJ (lane 1), His-FlgJ(E223Q) (lane 2), His-FlgJ(D248N) (lane 3), and His-FlgJ(E223Q-D248N) (lane 4).
