TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| E. coli | ||
| HMS174(DE3) | T7 expression host | 34 |
| S. typhimurium | ||
| KK2017 | flgJ::Tn10 | 25 |
| KK2057 | flgJ::Tn10 | 25 |
| KK2063 | flgJ::Tn10 | 25 |
| KK2083 | flgJ::Tn10 | 25 |
| KK2802 | flgJ::Tn10 | 25 |
| KK2813 | flgJ::Tn10 | 25 |
| KK2942 | flgJ::Tn10 | 25 |
| SJW1125 | flgJ1669 | 35 |
| SJW1139 | flgJ1683 | 35 |
| SJW1364 | flhA2014 | 24 |
| SJW1437 | flgJ2076 | 20 |
| SJW1456 | flgJ | 19 |
| SJW1488 | flgJ | 19 |
| Plasmids | ||
| pET19b | T7 expression vector, His tag, Apr | Novagen |
| pET-FLAG-19b | pET19b, His-FLAG tag | 8 |
| pLysS | T7 lysozyme, Cmr | 34 |
| pTrc99A | tac expression vector, Apr | 2 |
| pKK1490 | pBR322 flgIJKL+, Apr | 23 |
| pTN503 | pET19b His-FlgJΔN | This study |
| pTN504 | pET19b His-FlgJ | This study |
| pTN505 | pET19b His-FlgJ(E223Q) | This study |
| pTN506 | pET19b His-FlgJ(D248N) | This study |
| pTN507 | pET19b His-FlgJ(E223Q-D248N) | This study |
| pMM502 | pET-FLAG-19b His-FLAG-FlgJ | This study |
| pMM505 | pTrc99A His-FLAG-FlgJ | This study |