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. Author manuscript; available in PMC: 2023 Feb 3.
Published in final edited form as: Cancer Res. 2022 Aug 3;82(15):2678–2691. doi: 10.1158/0008-5472.CAN-22-0656

Figure 2: Hematopoietic Injury Decreases with Increasing Radiation Energy at a Constant Dose.

Figure 2:

Complete blood count of peripheral blood analyzed by one-way ANOVA for (A) hematocrit, (B) white blood cells, and (C) platelets 10-days after 7 Gy whole-body irradiation. Results combined from two independent experiments (n≥3/group). (D) Femoral cell counts were performed 10-days post-irradiation using an automated hemacytometer and combined from two independent experiments for analysis with one-way ANOVA. (E) H&E-stained sternal bone marrow depicting sternebrae 10-days post-irradiation representative of three independent experiments. (Scale Bar 100 μm; n≥6/group). (F) H&E-stained sternal bone marrow 10-days post-irradiation representative of three independent experiments (Scale Bar 20 μm; n≥6/group). (G) Prussian blue staining with nuclear fast red counterstain to detect iron (blue) in sternal bone marrow 10-days post-irradiation. Representative of three independent experiments (Scale Bar 25 μm; n≥6/group). (H) Hematopoietic stem and progenitor cell flow cytometry was performed on bone marrow collected from mice 10-days post-irradiation with results combined from two independent experiments analyzed by two-way ANOVA (n≥6/group). Populations are represented as the total number of cells per femur using logarithmic scale. Data is represented as mean ± SEM. P<.01 (**), P<.0001 (****). LT-HSC: Long-Term Hematopoietic Stem Cell, MPP: Multipotential Progenitor, CMP: Common Myeloid Progenitor, GMP: Granulocyte-Macrophage Progenitor, MEP: Myeloid-Erythroid Progenitor, CLP: Common Lymphoid Progenitor.