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. Author manuscript; available in PMC: 2023 Feb 3.
Published in final edited form as: Cancer Res. 2022 Aug 3;82(15):2678–2691. doi: 10.1158/0008-5472.CAN-22-0656

Figure 4: Gastrointestinal Injury is Dependent on Energy of Irradiation.

Figure 4:

(A) Gross pathology of stomachs 10-days after whole-body irradiation demonstrating gastric hemorrhage. (B) Gross gastric bleeding was scored semi-quantitatively by a blinded observer. (C) Representative H&E-stained sections of stomachs. Yellow arrowheads indicate mucosal hemorrhage. Yellow asterisk indicates submucosal hemorrhage. (Scale Bar 50 μm; n≥4/group). (D) H&E-stained intestinal sections from mice 4-days post-12 Gy WBI representative of two independent experiments. (Scale Bar 100 μm; n≥3/group.) (E) Total counts of crypts per circumference of intestinal cross-sections and (F) Crypt depth measurements 4-days post-irradiation analyzed by one-way ANOVA. (G) EdU incorporation was fluorescently detected (green) to assess cell proliferation in intestinal crypts 4-days post-12 Gy WBI. Images representative from two independent experiments. (Scale Bar 100 μm; n≥3/group). (H) EdU+ cells per intestinal crypt were counted 4-days post-irradiation and analyzed by one-way ANOVA. (I) Olfactomedin-4 (OLFM4; red) was detected by IHC 4-days after 12 Gy of whole-body irradiation, representative of two independent experiments (Scale Bar 100 μm; n≥3/group). (J) The percent of regenerating crypts (≥5 EdU+ cells/crypt) was calculated 4-days post-irradiation and analyzed by one-way ANOVA. Data is represented as mean ± SEM. P<.05 (*), P<.01 (**), P<.001 (***), P<.0001 (****).