Table 1.

Phenotypic and genotypic characteristics of the K. pneumoniae strain PM27 and its transconjugants.

Strain ID	Bacterial species	ST type	MIC (μg/ml) a													rmpA	Conjugation efficiency
			AMP	CAZ	CTX	MEM	GEN	AMK	AZI	CIP	CHL	TET	TIG	CLS	SPE
PM27	K. pneumoniae	66	8	0.5	0.06	0.06	0.5	1	2	0.008	4	2	0.5	2	16	+	NA
PM27-spe	K. pneumoniae	66	8	0.5	0.06	0.06	0.5	1	2	0.008	4	2	0.5	2	>128	+	NA
EC600	E. coli	NA	8	0.5	0.125	0.06	1	2	2	0.008	4	2	0.5	0.5	8	–	NA
EC600-TC	E. coli	NA	8	0.5	0.125	0.06	1	2	2	0.008	4	2	0.5	0.5	>128	+	1.25E-05
EH12PC	K. pneumoniae	23	64	0.25	0.06	0.06	0.5	1	4	0.008	2	2	0.5	2	8	–	NA
EH12PC-TCb	K. pneumoniae	23	64	0.25	0.06	0.06	0.5	1	4	0.008	2	2	0.5	2	>128	+	2.34E-08
PM8PC	K. pneumoniae	374	128	0.5	0.06	0.06	0.5	1	4	0.008	2	2	0.5	2	8	–	NA
PM8PC-TC	K. pneumoniae	374	128	0.5	0.06	0.06	0.5	1	4	0.008	2	2	0.5	2	>128	+	1.04E-08
14WZ-1	K. pneumoniae	11	>128	>128	>128	>128	0.5	1	4	>128	4	2	0.5	2	16	–	NA
14WZ-1-TC	K. pneumoniae	11	>128	>128	>128	>128	0.5	1	4	>128	4	2	0.5	2	>128	+	4.36E-08
25922	E. coli	NA	2	0.12	0.06	0.016	1	2	2	0.008	4	0.5	0.25	0.5	8	NA	NA

^aAMP, ampicillin; CAZ, ceftazidime; CTX, cefotaxime; MEM, meropenem; GEN, gentamicin; AMK, amikacin; AZI, azithromycin; CIP, ciprofloxacin; CHL, chloramphenicol; TET, tetracycline; TIG, tigecycline; CLS, colistin; SPE, spectinomycin. All tests were performed in duplicate, and each test included three biological replicates.

^bTransconjugants EH12PC-TC, PM8PC-TC, and 14WZ-1-TC were not subjected to spectinomycin-resistance-encoding cassette excision by Flp.