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. Author manuscript; available in PMC: 2023 Jun 20.
Published in final edited form as: Curr Biol. 2022 May 19;32(12):2704–2718.e6. doi: 10.1016/j.cub.2022.04.088

Key Resources table

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Anti-GFP ABCAM Cat# ab290
Anti GAPDH Cell Signaling Cat# 3675 S
Anti-6xHis Qiagen Cat# 34650
Anti-GST Cell Signaling Car# 2622S
Anti-GM-130 Cell Signaling Cat# 12480S
Anti-P-FAK-Y397 ThermoFisher Cat# 44-624G
Anti-FAK Antibody, clone 4.47 Millipore Cat# 05-537
Anti- MARK2 C-terminal ABCAM Cat# ab136872
Anti-MARK1 Cell Signaling Cat# 3319S
Anti-MARK3 ABCAM Cat# Ab 52626
Anti p-MLC S19 Cell Signaling Cat# 3675S
Anti-Myc tag Cell Signaling Cat# 2276
Anti-MYPT1 Millipore Cat# 07-672-1
Anti P-MYPT T696 Millipore Cat# AB545
Anti-P-MYPT T853 Cell Signaling Cat# 4563S
Anti-Myosin IIA (MYH9) Sigma Cat# M8064
Anti-alpha tubulin clone DM1A Sigma Cat# 05-829
HRP-conjugated goat anti-mouse Jackson ImmunoResearch Cat# 115-035-174
HRP-conjugated Donkey anti-mouse Jackson ImmunoResearch Cat# 112-035-175
Bacterial and virus strains
MAX Efficiency™ DH5α Competent Cells ThermoFisher Cat# 18258012
Chemicals, peptides, and recombinant proteins
MARK2, Active SignalChem Cat# M44-10G
MARK3, Active SignalChem Cat# M45-10G
ROCK2, Active SignalChem Cat# R11-RH-10
MLCK, Active 89 N/A
His-MRLC 90 N/A
Calmodulin .89 N/A
CHKtide SignalChem Cat# C10-58
MRLC peptide SignalChem Cat# C09-58
MYPT1, (fragment 654-880) Millipore Sigma Cat# 12-457
PhosSTOP Roche Cat#04906845001
PrecipHen Aves Lab Cat# P-1010
Native myosin (chicken gizzard) 91 N/A
Purified MRLC (chicken gizzard) 91 N/A
S-protein agarose Merck Millipore Cat# 69704
PhosSTOP Phosphatase inhibitors (Roche) Millipore/sigma Cat# 4906845001
cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail (Roche) Millipore/Sigma Cat# 11836170001
Tween-20 Sigma Cat# P1379
Fetal Bovine Serum Atlanta Biologicals-Biotechme Cat# S11150
McCoy’s 5A ThermoFisher Cat# 36600021
EC-Oxyrase OXYRASE Cat# EC-0005
Radiance ECL Azure
Biosystems		 Cat# AC2204
Alexa Fluor 647 NHS esther ThermoFisher Cat# A20006
Human Plasma Fibronectin Millipore Cat# FC010
Alexa Fluor 488 Phalloidin ThermoFisher Cat# A12379
Alexa 568 Phalloidin ThermoFisher Cat# A12380
Critical commercial assays
EndoFree Plasmid Maxi Kit Qiagen Cat# 12362
Primo system (micropattering) Alveole (Paris) N/A
SE Cell Line 4D-Nucleofector X Kit V Lonza Cat #: VACA-1003
GFP-Trap magnetic Agarose Chromotek Cat# gtma-20
XtremeGene9 Roche Cat# 6365779001
QuikChange II XL Agilent Technologies Cat# 200521
Experimental models: Cell lines
U2OS ATCC Cat# HTB-96
U2OS MARK2-KO This study N/A
U2OS MARK2-KO-MARK2-EGFP This study N/A
U2OS MARK2-KO-MARK2-DKA-EGFP This study N/A
U2OS MARK2-KO-MARK2-mApple This study N/A
Cos1 ATCC Cat# CRL-1650
Oligonucleotides
On Target plus Human MARK3 SiRNA Dharmacon (Horizon) Cat# L-00357-00-0020
On Target plus Human MARK2 SiRNA Dharmacon (Horizon) Cat# L-004260-00-0020
MARK2-KO Guide 1
(GGAGCCGGTAGTTTCCAATG )		 This paper N/A
MARK2-KO Guide2 (GTGTCGGGCCAACTTCACCT) This paper N/A
MARK2-KO Guide 3(AGCCCCACATTGGAAACTAC) This paper N/A
Recombinant DNA
MARK2-EGFP 38 N/A
MARK2-mApple 38 N/A
MARK2-DKA-EGFP This paper N/A
pGFP-MYPT1 92 N/A
pTriEX4-MARK2 This project N/A
pTriEX4 MERK Sigma 70824
Myosin IIA-GFP Michael Davidson Lab Florida State University N/A
EGFP-C1 Michael Davidson Lab Florida State University N/A
m-Apple-C1 Michael Davidson Lab Florida State University N/A
Paxillin-GFP Michael Davidson Lab Florida State University N/A
pSpCas9n(BB)-2A-Puro (PX462) V2.0 Addgene Cat# 62987
Software and algorithms
Metamorph 7.8.0.0 ID:32415 1992-2013 https://www.moleculardevices.com/products/cellular-imaging-systems/acquisition-and-analysis-software/me
GraphPad Prism 9.0.2 2021 https://www.graphpad.com/scientific-software/prism/
Other
Novex Wedgewell 4–12 % tris-Glycine gel Invitrogen ThermoFisher Cat# XP04120BOX
Novex Wedgewell 4–20 % tris-Glycine gel Invitrogen ThermoFisher Cat# XP04202BOX