FIGURE 2.

Effect of reaction conditions on glycosylation yields from low-T _m enzymes. Analytic yields of acceptor conversion are plotted against enzyme concentration, from 10 to 80 mg/L (circa 0.15–1.2 μM). HEPES pH 7, aglycon concentration range 50–400 μM, UDP-Glc 500 μM, 20 h at 293 K, without stirring in 100 μl volume. The 9 GT1s with the lowest T _m or no measured T _m are displayed. NA = Not Available, as the fluorescence as a function of the temperature did not reflect a classical behavior melting temperature.