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Effect of reaction conditions on glycosylation yields from high-T m enzymes. Analytic yields of acceptor conversion are plotted against enzyme concentration, from 10 to 80 mg/L (circa 0.15–1.2 μM). HEPES pH 7, aglycon concentration range 50–400 μM, UDP-Glc 500 μM, 20 h at 293 K, without stirring in 100 μL volume.
