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Calibration of changes in (i) PL spectra, (ii) FRET efficiency (from donor quenching), and (iii) A/D PL ratio with an increasing number of (A) Cy3- or (B) A594-labeled peptide substrates and products. The inset in B(i) shows the direct excitation of A594. * Designates mixtures of native and digested peptide with a constant total valence of 12 for the QD-A549 system.
