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. Author manuscript; available in PMC: 2022 Aug 5.
Published in final edited form as: Nano Lett. 2012 Jun 25;12(7):3793–3802. doi: 10.1021/nl301727k

Figure 4.

Figure 4.

Progress curves in (i) normal time and (ii) enzyme-time for hydrolysis of QD-(peptide-Cy3/A594)n conjugates, catalyzed by different trypsin concentrations: (A) n = 2.4 (Cy3), (B) n = 3.7 (Cy3), (C) n = 7.0 (Cy3), and (D) n = 13.4 (A594). Trypsin concentrations were 0, 1.3, 2.7, 5.4, 11, 22, 43, 86, 172, 343, 687 nM, except for n = 2.4, where 687 nM was replaced with 0.6 nM. In (ii), blue curves are best fits to the MM model, and red curves are those predicted from the values of Km and V for only the peptide substrate in bulk solution.