Table 1.

Relevant kinetic parameters for QD-peptide substrate conjugates, bulk peptide substrate and BANA.

Substrate		$k_{\text{cat}}\left({\text{s}}^{-1}\right)$	$K_{\text{m}}(\mu \text{M})$ a	$k_{\text{cat}}/K_{\text{m}}\left({\text{mM}}^{-1}{\text{s}}^{-1}\right)$ b
CSTRIDEANQAATSLP7SH6 (peptide)		0.53 ± 0.03	434 ± 53	1.2 ± 0.2
QD Conjugates	[QD] (μM)	[S] (μM)	$K_{\text{m},\text{eff}}(\mu \text{M})$	$k_{\text{cat}}/K_{\text{m}}\left({\text{mM}}^{-1}{\text{s}}^{-1}\right)$
QD-(peptide-Cy3)2.4	0.5	1.2	95 ± 5	5.6 ± 0.3
QD-(peptide-Cy3)3.7	0.5	1.8	160 ± 10	3.3 ± 0.2
QD-(peptide-Cy3)7.0	0.5	3.5	146 ± 9	3.6 ± 0.2
QD-(peptide-A594)13.4	0.5	6.7	119 ± 7	4.4 ± 0.3

^aThe uncertainty in $K_{\text{m}}$ is derived from the uncertainty in $k_{\text{cat}}/K_{\text{m}}$.

^bThe listed uncertainties are standard errors associated with curve fitting the average data from three replicate experiments.