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Gradient program of the mobile phase for HPLC separation of sweeteners in the present study.
| Time (min) | Flow rate (mL/min) | Mobile phase | |
|---|---|---|---|
|
| |||
| Aa (%) | Bb (%) | ||
| 0 | 1.0 | 80 | 20 |
| 3 | 1.0 | 80 | 20 |
| 6 | 1.0 | 50 | 50 |
| 12 | 1.0 | 0 | 100 |
| 15 | 1.0 | 0 | 100 |
| 15.5 | 1.0 | 80 | 20 |
| 18 | 1.0 | 80 | 20 |
HPLC = high performance liquid chromatography.
A: 10 mM ammonium acetate in deionized water.
B: 10 mM ammonium acetate in methanol.
