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. 2022 Jul 22;13:939907. doi: 10.3389/fimmu.2022.939907

Figure 1.

Figure 1

MiR-3614-5p is induced by type I IFN. (A) MiR-3614-5p expression in monocytes purified from two healthy donors and treated with 100pM IFN-β for 24 hr (black) or not treated (white). Results are shown as expression (2-ΔCt) relative to U6 used as housekeeping gene. Values are means ± SEM. (B) Mir-3614-5p expression in monocytes from one donor and in derived macrophages (Mo-Mϕ) and dendritic cells (Mo-DC). IFN-β stimulation and results as in A). (C) Mir-3614-5p expression in different human cell lines and in primary monocytes from a single donor (Mo). Stimulation with IFN-α (100pM) for 8 hr. Data are representative of two independent experiments. ND, not detected. (D) MiR-3614-5p (left panel) and TRIM25 (right panel) levels measured in primary monocytes stimulated with IFN-β (100pM) as indicated. Results for TRIM25 are shown as expression (2-ΔCt) relative to 18S. Data are representative of two independent experiments. (E) MiR-3614-5p (left panel) and TRIM25 (right panel) levels measured in HeLa cells stimulated with IFN-α (100pM) for the indicated times. Results expressed as in D). Data are representative of four independent experiments.