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Auxotrophic requirement of ICR-191-induced mutants of strain AS18-29a
| Strain | Requirement | Locus | No. of revertants/ 108 cells
|
|
|---|---|---|---|---|
| WT | mutH | |||
| AS201 | Cysteine | cys | 300 | ∼10,000 |
| AS210 | Leucine | leu | 40 | ∼5,000 |
| AS212 | Isoleucine | ile | 700 | ∼10,000 |
| AS213 | Arginine | arg | 20 | ∼10,000 |
| AS220 | Adenine | pur | 5 | ∼10,000 |
Each of the auxotrophic requirements is in addition to the Met− phenotype of AS18-29 (see legend to Fig. 1). WT, wild type.
