Table 1.
Effects of methanolic extract of Dolichos biflorus seeds on malondialdehyde and antioxidants.
| Groups | I | II | III | IV |
|---|---|---|---|---|
| Malondialdehyde (mM/g tissue) | 1.60 ± 0.09 | 4.50 ± 0.28a | 2.80 ± 0.14a,b | 2.30 ± 0.10a,b |
| Glutathione (mM/g tissue) | 18 ± 1.52 | 6 ± 0.91a | 12 ± 0.10a,b | 9 ± 0.13a,b |
| Catalase (Units*/mg protein) | 4.80 ± 0.37 | 2.10 ± 0.13a | 2.90 ± 0.16a,b | 2.60 ± 0.11a,b |
| Superoxide dismutase (Units$/mg protein) | 3.80 ± 0.03 | 1.30 ± 0.09a | 2.50 ± 0.14a,b | 2.20 ± 0.10a,b |
| Glutathione peroxidase (Units#/mg protein) | 10.50 ± 0.93 | 6.60 ± 0.79a | 8.02 ± 0.68a,b | 7.50 ± 0.63a,b |
| Glutathione reductase (Units#/mg protein) | 480.08 ± 7.7 | 100.14 ± 2.8a | 360.25 ± 9.1a,b | 320.93 ± 5.6a,b |
| Vitamin C (mg/100 g tissue) | 100.33 ± 1.22 | 38.56 ± 2.53a | 50.12 ± 0.962a,b | 46.72 ± 0.31a,b |
| Ceruloplasmin (mg/dL) | 0.08 ± 0.001 | 0.66 ± 0.005a | 0.21 ± 0.002a,b | 0.30 ± 0.001a,b |
Values expressed as average of six rats ± standard deviation in each group. Group I: normal; Group II: rats were given 0.1 mL 1% carrageenan; Group III: rats were given 0.1 mL 1% carrageenan + methanolic extract of Dolichos biflorus seeds; and Group IV: Rats were given 0.1 mL 1% carrageenan + Voveran.
a
= statistical difference compared with Group I at p < 0.05;
b
= statistical difference when Group II compared with Groups III and IV at p < 0.05.
*
Velocity constant/second;
$
enzyme concentration to inhibit chromogen production (OD 560 nm) by 50% in 1 minute;
#
1 μmol NADPH oxidized/min/mg protein.