Table 1.

Effects of methanolic extract of Dolichos biflorus seeds on malondialdehyde and antioxidants.

Groups	I	II	III	IV
Malondialdehyde (mM/g tissue)	1.60 ± 0.09	4.50 ± 0.28a	2.80 ± 0.14a,b	2.30 ± 0.10a,b
Glutathione (mM/g tissue)	18 ± 1.52	6 ± 0.91a	12 ± 0.10a,b	9 ± 0.13a,b
Catalase (Units*/mg protein)	4.80 ± 0.37	2.10 ± 0.13a	2.90 ± 0.16a,b	2.60 ± 0.11a,b
Superoxide dismutase (Units$/mg protein)	3.80 ± 0.03	1.30 ± 0.09a	2.50 ± 0.14a,b	2.20 ± 0.10a,b
Glutathione peroxidase (Units#/mg protein)	10.50 ± 0.93	6.60 ± 0.79a	8.02 ± 0.68a,b	7.50 ± 0.63a,b
Glutathione reductase (Units#/mg protein)	480.08 ± 7.7	100.14 ± 2.8a	360.25 ± 9.1a,b	320.93 ± 5.6a,b
Vitamin C (mg/100 g tissue)	100.33 ± 1.22	38.56 ± 2.53a	50.12 ± 0.962a,b	46.72 ± 0.31a,b
Ceruloplasmin (mg/dL)	0.08 ± 0.001	0.66 ± 0.005a	0.21 ± 0.002a,b	0.30 ± 0.001a,b

Values expressed as average of six rats ± standard deviation in each group. Group I: normal; Group II: rats were given 0.1 mL 1% carrageenan; Group III: rats were given 0.1 mL 1% carrageenan + methanolic extract of Dolichos biflorus seeds; and Group IV: Rats were given 0.1 mL 1% carrageenan + Voveran.

^a= statistical difference compared with Group I at p < 0.05;

^b= statistical difference when Group II compared with Groups III and IV at p < 0.05.

^*Velocity constant/second;

^$enzyme concentration to inhibit chromogen production (OD 560 nm) by 50% in 1 minute;

^#1 μmol NADPH oxidized/min/mg protein.