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. 2022 Jul 22;13:945411. doi: 10.3389/fmicb.2022.945411

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Copyright © 2022 Johannessen, Anthonisen, Zecic, Hegstad, Ranheim and Skaare.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Illustration of the insertion point of Tn7100 in Hi-228, which is located in a tRNA^Leu gene. The insertion of Tn7100 results in a complete tRNA^Leu gene (AP022867.1) at the left attachment site (attL) and a complete trnL gene (AF467992.1) at the right attachment site (attR). (B) Illustration of excision, circularization, transfer, and integration of Tn7100 from Hi-228 to the recipient strain, as confirmed by experimental procedures in this study.